Fig. 2. The Hes.a repressor forms puncta in Ciona embryos dependent on DNA binding and the presence of a WRPW domain.

a Maximum intensity confocal projections of ~110-cell stage embryos expressing Hes.a fusion proteins. Cell membranes are colored magenta and Hes.a::mNg fusion proteins are green. The embryos are oriented to show the animal hemisphere, anterior left. Scale bar = 20 μm. b Confocal images of individual nuclei expressing Hes.a proteins fused to mNg. Single confocal sections are shown in white, color coded projections are shown with the indicated look up table. c Same as b but for the Snai::mNg. Scale bar = 1 μm. All transgenes were expressed using pSP Sox1/2/3 > plasmids. All images are representative of >3 biological replicates.