Fig. 3. Hes.a represses ZicL.

a Red fluorescence in in gastrula stage Ciona embryos that have been from a ZicL > H2b::mC reporter plasmid. The embryos have been coelectroporated with Hes.a fusion proteins that were expressed earlier in development using plasmids containing the Sox1/2/3 regulatory region. b Merged green and red fluorescent channel from the image in a. Because most of the Hes.a proteins have been degraded by this stage, cells that were previously expressing Hes.a transgenes were determined by the presence of the membrane localized mNG::PH fusion protein expressed using the Sox1/2/3 regulatory region. Scale bar = 20 μm. All images are representative of >3 biological replicates.