Fig. 4. Hes.a/Groucho puncta are distinct molecular condensates.
a Maximum intensity projection confocal images of single Ciona nuclei expressing Hes.a::mNg. a’ mAp::Gro Fluorescence. a” The merged green and red channels for a and a’. Scale bar = 1 μm. b–b”, As for the a series but for Hes.a E22V,R28C::mNG and mAp::Gro Green and red channels are shown individually and merged. c–c”, Hes.a::mNg and Cdyl:mCh d-d”, Hes.a::mNg and Hp1::mAp. c Pearson correlation coefficients of the experiments shown in a–d”. Boxes display the mean as the central line and the limits of the box denote standard deviations. All transgenes were expressed using pSP Sox1/2/3 > plasmids. All images are representative of >3 biological replicates. Source data are provided as a Source Data file.