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. 2021 Mar 10;12:1561. doi: 10.1038/s41467-021-21606-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Time-lapse maximum intensity projection confocal images of region of a single Hes.a puncta. Images show the puncta prior to photobleaching and the recovery at several timepoints. Fluorescent intensity is indicated by the look up table. Scale bar = 1 μm. b Quantification of FRAP recovery of Hes.a droplets. Dots indicate the mean and the error bars indicate the standard deviation (N = 3). c Time-lapse maximum intensity projection confocal images of Hes.a puncta. 2 puncta are indicated with arrows and if they fuse are indicated by a single arrow. Scale bar = 0.5 μm. All transgenes were expressed using pSP Sox1/2/3 > plasmids. FRAP and fusion images are representative of >3 biological replicates. Source data are provided as a Source Data file.