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. 2021 Mar 10;11:5552. doi: 10.1038/s41598-021-84943-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RXRγ KO mice exhibit impaired group 1 mGluR-activated voltage-sensitive currents. (A) Schematic of voltage-clamp stimulation ramp to elicit voltage-sensitive inward currents. (B) Sample current/voltage relations in a wild type CA1 pyramidal neuron during bath-application of the group I agonist DHPG (30 µM, red trace, subtraction of pre-drug baseline I/V plot from I/V relation after 20 min application of DHPG) compared to washout control (black trace) in the same cell. (C) Sample current/voltage relations in an RXRγ KO CA1 pyramidal neuron during bath-application of DHPG (30 µM, red trace, subtraction of pre-drug baseline I/V plot from I/V relation after 20 min application of DHPG) compared to washout control (black trace) in the same cell. (D) Sample inward currents in a wild type CA1 pyramidal neuron before (black trace), during (red trace), and after (blue trace) bath application of 30 µM DHPG (WT peak control current: 40.8 ± 11.4 pA; peak current in presence of DHPG: 21.7 ± 9.6 pA, N = 7 cells, (paired t-test; t = 2.788, P = 0.0317). (E) Sample inward currents in an RXRγ KO CA1 pyramidal neuron before (black trace), during (red trace), and after (blue trace) bath application of 30 µM DHPG (KO peak control current: 37.3 ± 16.5 pA; peak current in presence of DHPG: 42.5 ± 17.6 pA, N = -6 cells, (paired t-test; t = 0.552, P = 0.6044). (F) DHPG (30 µM, grey bar) reduced voltage-sensitive inward currents, plotted as reduction in membrane input resistance Rm, in wild type control neurons. (G) DHPG (30 µM, grey bar) did not alter voltage-sensitive inward currents in CA1 pyramidal neurons in slices from RXRγ KO mice. Data were plotted using Origin Pro (https://www.originlab.com) and the figure assembled using Affinity Designer (https://affinity.serif.com/en-gb/designer/) software.