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. 2021 Mar 10;9:16. doi: 10.1038/s41413-020-00124-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Expression of Piezo1 in MC3T3-E1 and shRNA-Piezo1 MC3T3-E1 cells. a Piezo1 (green) was expressed on MC3T3-E1 cells and localized at the plasma membrane and nucleus (blue). The protein expression of Piezo1 was decreased in shRNA-Piezo1 cells, especially on the plasma membrane. Piezo1 was expressed only around the nucleus in the shRNA-Piezo1 cells. The scale bar is 50 μm. b, c Western blot analysis showed that the Piezo1 protein expression in the shRNA-Piezo1 MC3T3-E1 group was significantly lower than that in the control group (n = 3, P < 0.01, Student’s t-test)