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. 2021 Mar 10;9:16. doi: 10.1038/s41413-020-00124-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Western blot analysis of ERK1/2 and p-ERK1/2 in MC3T3-E1 and shRNA-Piezo1 cells after LIPUS stimulation. a Representative western blots of ERK1/2, p-ERK1/2, and GAPDH in MC3T3-E1 and shRNA-Piezo1 cells at the indicated time points (0, 5, and 30 min) after LIPUS stimulation. b Quantitative changes in ERK1/2 activation in MC3T3-E1 and shRNA-Piezo1 cells. The ratio of ERK1/2 phosphorylation to the relative expression of the protein doublet (p-ERK1/2 vs. ERK1/2) is presented as a parameter of ERK1/2 activation (n = 3, *P < 0.05, **P < 0.01, Student’s t-test)