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. 2021 Mar 10;12:1556. doi: 10.1038/s41467-021-21661-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Genomic distance distribution of averaged DNA methylation ratios in MO and moDC across “TF peak”, “open”, and “no peak” groups (in blue, yellow, and red, respectively, as defined in Fig. 3c) of DMR demethylated in moDC. DMR were centered on the consensus EGR (left panel) or AP1 motifs (right panel), which are shown on top of each panel (CpG positions that are highlighted by arrows in gray (MO) and black (moDC) in the lower panels are circled). Each panel shows the distribution of DNA methylation ratios across a 1-kb window (large graph) as well as a 12-bp window focusing on the motif-covered CpGs only (smaller inlay graph in the bottom right corner). The number of motifs containing DMR is given in the lower left corner of each panel. b IGV genome browser tracks of example loci showing single CpG methylation ratios (WGBS), 5hmC and ATAC-seq coverage in MO and moDC, and EGR2 ChIP-seq coverage in moDC. Genomic locations of DMR are shown (coloring according to the classification into DMR groups “no peak” or “TF peak” in red or blue, respectively). EGR motif sequences are given below each track and arrows in the top tracks mark the positions of the circled CpG. c CoIPs of EGR2 or NAB2 (expressed as FLAG-tagged proteins via mRNA electroporation) and TET2 in THP-1 and moDC. Results are representative of two independent experiments. d Model of EGR2-induced DNA demethylation at transient and stable binding sites in moDC. (a, c) Source data are provided as a Source Data file.