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. 2021 Mar 10;11:5573. doi: 10.1038/s41598-021-84663-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differential gene expression (DGE) of miRNAs of apical and basal spikelets in V2 (SR-157) over V1 (SR-159) during post-anthesis (0, 5 and 10 days) using DESeq8 tool (a). DGE of miRNAs of V2 (SR-157) over V1 (SR-159) based on read length showing UP-regulation or DOWN-regulation after anthesis on 0 day in apical spikelets (b), 0 day in basal spikelets (c), 5 days in apical spikelets (d), 5 days in basal spikelets (e), 10 days in apical spikelets (f), 10 days in basal spikelets (g). Heat map showing DGE analysis of selected miRNAs in apical and basal spikelets of V1 (SR-159) and V2 (SR-157) on days 0, 5 and 10 after anthesis. Here, Log₂ fold change of 1 was used as cut off, miRNAs > 1 were considered “UP- (red colour code)” regulated, miRNAs < -1 considered “DOWN- (green colour code)” regulated and those between 1 and − 1 were considered as “NEUTRAL (black colour code)” (h).