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. 2021 Mar 10;4:323. doi: 10.1038/s42003-021-01858-z

Fig. 3. Effects of adipocyte-specific HSL deletion on hepatic lipid homeostasis.

Fig. 3

a Hepatic triglyceride (TG) content (M, 10 and 26 weeks, HFD, ad libitum fed, n = 6–10 animals/genotype). b Liver mRNA expression of immune cell markers and genes involved in fibrosis relative to Cyclophilin reference gene by qPCR with control animals arbitrarily set to 1 for each gene (M, 26 weeks, HFD, ad libitum fed, n = 5–6 animals/genotype). c 12 h fasted and refed plasma fatty acid (FA; left) and insulin (right) levels (M, HFD, 17 weeks, n = 4–7 animals/genotype). d Insulin signaling in WAT. Mice were injected with saline or insulin at 0.75 IU kg−1 body weight. Left: quantification of phosphorylation of AKTpSer473/total AKT in PGAT and SCAT (M, 26 weeks, HFD, 12 h fasted, n = 3 animals/genotype). Right: representative immunoblots. e Hepatic mRNA expression of FA transporter in the liver (M, 22 weeks, HFD, ad libitum fed, n = 6–8 animals/genotype) and f genes involved in de novo lipogenesis and triglyceride (TG) synthesis relative to Cyclophilin reference gene by qPCR with control animals arbitrarily set to 1 for each gene (M, 26 weeks, HFD, ad libitum fed, n = 5–6 animals/genotype). g Left: liver expression of proteins involved in de novo lipogenesis (SREBP1c, FAS, SCD1). Right: representative immunoblots (M, 26 weeks, HFD, ad libitum fed, n = 6 animals/genotype). h Left: liver protein expression of lipolytic proteins (HSL, ATGL, ABHD5). Right: representative immunoblots (M, 26 weeks, HFD, ad libitum fed, n = 6 animals/genotype). i Triglyceride hydrolase activity in liver determined by [3H]-labeled oleic acid release (M, 26 weeks, HFD, ad libitum fed, n = 4–5 animals/genotype). Data represent mean + SEM. Statistical significance was determined by Student’s two-tailed t test. P < 0.05: * for effect of genotype; # for effect of age; & for effect of treatment.