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. 2021 Mar 10;12:1565. doi: 10.1038/s41467-021-21865-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a A total of six clusters of stromal cells were identified in developing lungs. Cell populations are colored as indicated by the legend. b UMAP plots showing expression of principal identifiers of different stromal cell types. The intensity of expression is indicated by purple coloring. c Heatmap of top five most differentially expressed genes across stromal clusters. The intensity of expression is indicated as specified by the color legend. d UMAP plots depicting cell identity in regard to developmental time points in normally (purple) and aberrantly (green) developing lung endothelium. The intensity of expression is indicated as specified by the color legend. e Dotplot depicting expression of oxygen-specific markers in Col13a1⁺ fibroblasts at P14. The intensity of expression is indicated by the color legend. Size of the cell population expressing the gene of interest is indicated by the size of the circle as specified by the legend. f Fluorescent RNA in situ hybridization showing co-expression of Inmt (pink) and Saa3 (pink) with Col13a1 (green) in normal and aberrant mouse lungs. Magnification: 40×. Scale bar = 40 µm. Two 14-days old animals/group were analyzed. g Dotplot depicting expression of oxygen-specific markers in Myofib. cluster at P14. The intensity of expression is indicated by the color legend. Size of the cell population expressing the gene of interest is indicated by the size of the circle as specified by the legend. h Selected hyperoxia-impacted signaling pathways in Col13a1⁺ fib (pink), Myofib. (yellow), and Pericytes 2 (blue) clusters as identified by gene set enrichment analysis (GSEA). All terms are significantly enriched (adjusted p value < 0.05) and normalized enrichment scores (NES) are shown. NES values were computed by gene set enrichment analysis on fold change-ranked genes. Expression values in Heatmap and violin plots represent Z-score-transformed log(TP10k + 1) values. Expression levels in UMAP plots and Dotplots are presented as log(TP10k + 1) values. Log(TP10k + 1) corresponds to log-transformed UMIs per 10k.