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. 2021 Mar 10;11:5540. doi: 10.1038/s41598-021-84785-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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DAAM2 mRNA and protein in preterm placental tissue (≤ 34 weeks). (a) DAAM2 mRNA expression assessed by qPCR. (b) Representative western blot and (c) densitometric analysis of DAAM2 protein. DAAM2 mRNA expression was not significantly changed in fetal growth restricted (FGR; n = 14) tissue compared to preterm control (PT; n = 10) tissue. Relative levels of DAAM2 protein were significantly higher in fetal growth restricted (n = 16) placental tissue compared to preterm control (n = 9) tissue. Protein and mRNA data presented as fold change from control ± SEM. *p < 0.05. Full western blot images are presented in Supplementary Fig. S3.