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. 2021 Mar 10;11:5540. doi: 10.1038/s41598-021-84785-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of DAAM2 under normoxic (8% O₂) and hypoxic (1% O₂) conditions. Cytotrophoblast expression of DAAM2 mRNA was significantly knocked down under both normoxic (A) and hypoxic conditions with siRNA against DAAM2 (siDAAM2) compared to the negative control siRNA (siNegative). Data presented as fold change from control ± SEM. ****p < 0.0001. n = 3 experimental replicates (each sample from a different patient), with each experiment performed in triplicate.