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. 2021 Feb 25;11:621154. doi: 10.3389/fonc.2021.621154

Figure 5.

Figure 5

LRIG3 modulates VEGFA expression via the PI3K/AKT signaling pathway. Western blots showing LRIG3, p-AKT, AKT, p-ERK, ERK, and VEGFA (A) alongside GAPDH (B) in the indicated glioma cells treated with the pathway inhibitors LY294002 (25μM), PD98059 (10μM), or DMSO vehicle for 24 h. (C) Levels of VEGFA protein in the CM from the indicated glioma cells were detected by ELISA. (D) Transwell migration assays in HUVECs treated with the indicated CM. (E) HUVECs formed tube-like structures with the indicated CM. (F) Cell viability of HUVECs was determined using the MTT assay. HUVECs were treated with the CM derived from the indicated cells for the specific number of days. Data are means ± SD of 3 independent replicates. *p < 0.05; **p < 0.01; ***p < 0.001; # p < 0.05, siLRIG3 + LY294002 versus siLRIG3 + vehicle and siLRIG3 + PD98059; ## p < 0.01, siLRIG3 + LY294002 versus siLRIG3 + vehicle and siLRIG3 + PD98059. CM, conditioned medium.