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. 2021 Feb 25;12:599296. doi: 10.3389/fimmu.2021.599296

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Copyright © 2021 Farazuddin, Landers, Janczak, Lindsey, Finkelman, Baker and O’Konek

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NE immunotherapy protects against allergic reactivity in polysensitized mice. (A) Mice were sensitized with OVA and peanut-alum and treated i.n. with 3 administrations of PBS (sensitized control) or OVA and peanut-NE (OVA+PN-NE) Mice were challenged orally with OVA and peanut and (B) symptoms of anaphylaxis, (C) diarrhea and (D) temperature change were monitored. (E) Hemoconcentration was determined by hematocrit. (F) Levels of MCPT-1 in the serum 60 min after challenge were determined by ELISA. Statistically significant differences (p < 0.05) are indicated by *.