Figure 2.

Immunization of polysensitized mice with NE and one allergen provides protection against reactivity to another allergen. As described in Figure 1A, mice were sensitized with OVA and peanut-alum and treated i.n. with 3 administrations of PBS (sensitized control), ova and peanut-NE (OVA+PN-NE), OVA-NE or peanut-NE (PN-NE). Mice were challenged orally with (A) OVA or (B) peanut and temperature change and symptoms of anaphylaxis were monitored. Hemoconcentration was determined by hematocrit. Levels of MCPT-1 in the serum 60 min after challenge were determined by ELISA. Statistically significant differences (p < 0.05) are indicated by *.