Figure 4.

NE immunotherapy induces antigen-specific and bystander suppression of Th2 cytokines and alarmins. As described in Figure 1A , mice were sensitized with OVA and peanut-alum and treated i.n. with 3 administrations of PBS (sensitized control), OVA-NE or peanut-NE (PN-NE). Mice were challenged orally with OVA, and (A) OVA-specific IgE was measured in serum obtained after the final challenge and (B) OVA-specific cytokine secretion was determined in cultures of mLN lymphocytes. (C) Duodenum samples were homogenized and mRNA extracted to determine relative gene expression compared to GAPDH. (D) Total number of ILC2 (Lin- CD45+ CD127+ CD90.2+ KLRG1+ GATA3+) from SI. Statistically significant differences (p < 0.05) are indicated by *.