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IFN-γ is required for suppression of alarmins by NE allergy vaccines. Mice were sensitized with OVA and peanut-alum and treated i.n. with 3 administrations of PBS (sensitized control) or peanut-NE (PN-NE). Mice were challenged orally with OVA and IFN- γ was depleted during the challenge phase. Duodenum samples were homogenized and mRNA was extracted to determine relative gene expression compared to GAPDH. Statistically significant differences (p < 0.05) are indicated by *.
