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. 2021 Feb 25;12:633171. doi: 10.3389/fphys.2021.633171

TABLE 1.

Sequences for all the primers (both forward and reverse) along with their respective purpose in the present study.

Gene name Sequence Purpose PCR product size Accession number
gucy1a1-F GTTTGGAGAGGACCATTACGC Sequencing 265 NM_001309529.1
gucy1a1-R TTTACCTCTGGACACACCAGC Sequencing
gucy1a1-F GGACAAAGATCCAGGGCTCC MO qRT-PCR 156 NM_001309529.1
gucy1a1-R AGAGCGCCACTGTCTTTACC MO qRT-PCR
gucy1b1-F AGTGTTGGGCTCCAATGTCC MO qRT-PCR 212 NM_001251945.2
gucy1b1-R CGTGAATCTGCTGTGCAACC MO qRT-PCR
cct7-F GGTGGGGCAATTGAGATGGA MO qRT-PCR 198 NM_173248.1
cct7-R CCACATACCACCCTGTGCAT MO qRT-PCR
EF1α-F CTGGAGGCCAGCTCAAACAT HKG 87 NM_131263.1
EF1α-R ATCAAGAAGAGTAGTACCGCTAGCATTAC HKG
β-actin-F CGAGCTGTCTTCCCATCCA HKG 86 NM_181601.5
β-actin-R TCACCAACGTAGCTGTCTTTCTG HKG