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. 2021 Mar 10;12(3):254. doi: 10.1038/s41419-021-03544-8

Fig. 3. Involvement of LXRα in CTRP12-induced upregulation of ABCA1 and ABCG1 expression.

Fig. 3

A THP-1 macrophages were treated with 50 µg/mL ox-LDL for 48 h, followed by transfection with PBS, LV-NC, or LV-CTRP12 for 72 h. LXRα expression was determined by qRT-PCR and western blot. B After transfection with scrambled siRNA or LXRα siRNA for 48 h, cell lysates were immunoblotted with indicated antibodies. C–E THP-1 macrophages were loaded with 50 µg/mL ox-LDL for 48 h, transfected with LXRα siRNA for another 48 h and then treated with LV-CTRP12 for 72 h. C The mRNA and protein levels of ABCA1 and ABCG1 were detected by qRT-PCR and western blot, respectively. D, E Representative fluorescent images of NBD-cholesterol burden (×200) and quantitative analyses of cholesterol efflux to apoA-I and HDL. Scale bar = 20 μm. Data are expressed as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.