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. 2021 Feb 25;11:586771. doi: 10.3389/fonc.2021.586771

Figure 1.

Figure 1

Rab12 was highly expressed in cervical cancer and oncoproteins E6 and E7 promoted the expression of Rab12. (A) Real-time PCR showed that Rab12 mRNA level was higher in clinical cervical cancer tissues (n=63) compared with non-cancer cervix tissues (n=46). (B) Kaplan-Meier curves for overall survival of 60 patients with cervical cancer according to Rab12 expression (Log-Rank test, P > 0.05). (C) Rab12 protein expression was analyzed by Western blot in HaCaT and cervical cancer cell lines HeLa, SiHa, Caski, C33A. (D, E) Western blot analysis of Rab12 proteins in HaCaT cells overexpressing 16E7 and Rab12, p53, E2F1 proteins in RPE1 cells overexpressing 16E6, 16E7; GAPDH was used as the loading control. (F–H) Protein levels of Rab12, p53, E2F1 in HeLa, SiHa, Caski cells after transfection with siRNA targeting 18E6, 18E6E7, or 16E6, 16E6E7 for 48 h; Comparable Western blots were observed in three independent experiments. Data are presented as mean ± SD from three independent experiments, **p < 0.01, ***p < 0.001.