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. 2021 Feb 25;11:586771. doi: 10.3389/fonc.2021.586771

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Copyright © 2021 Huang, Tian, Zhang, Liu and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Radiation promoted the expression of Rab12 at both mRNA and protein levels. (A) Western blot analysis of Rab12 expression in SiHa cells at different radiation doses (24 h). (B) Rab12 expression in SiHa cells at different times after radiation (6Gy). (C) Effects of radiation (6Gy, 24 h) on Rab12 expression in SiHa cells were determined by real-time PCR and Western blot analysis. (D) Immunofluorescent staining and comet assay showed the presence of nuclear debris and DNA breaks following exposure to 6Gy radiation. Comparable Western blots were observed in three independent experiments. Data are presented as mean ± SD from three independent experiments, ***p < 0.001.