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. 2021 Feb 25;11:586771. doi: 10.3389/fonc.2021.586771

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Copyright © 2021 Huang, Tian, Zhang, Liu and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rab12 alleviated DNA damage after radiation. (A, D) Double-strand breaks (DSBs) were analyzed using the neutral comet assay and immunofluorescent staining of γ-H2AX. Immunofluorescence showed that γ-H2AX was located in the nuclear of SiHa and C33A cells (red). There were more γ-H2AX foci in SiHa-ShRab12 cells than control cells, and γ-H2AX foci in C33A-pENTER-Rab12 cells decreased after exposure to radiation than control cells (6Gy, 24 h). DAPI (blue): nuclear counterstain. (B, E) The comet assay showed changes in tails in SiHa and C33A cells with Rab12 knockdown or overexpression after radiation. DNA was stained with DAPI (blue). The cells were visualized by fluorescence microcopy. (C, F) DSB repair proteins pBRCA1 and XRCC4 in Rab12 knockdown and overexpression cells after irradiation were detected by Western blot. Data are presented as mean ± SD from three independent experiments, *p < 0.5, **p < 0.01, ***p < 0.001.