Table 2.
The chemical analysis used in the experiment.
| Items | Methods | Assay kits/Testing equipment |
|---|---|---|
| Composition of ingredients | ||
| Amino acids (except tryptophan) | Acid hydrolysis | Amino acid analyzer: SYKAM S-433D (Sykam GmbH, Munich, Germany) |
| Tryptophan | Alkali hydrolysis | |
| Intestinal parameters related antioxidant capacity | ||
| SOD a | WST-1 method | Assay kits purchased from Jian Cheng Bioengineering Institute (Nanjing, China); Spectrophotometer (Thermo Fisher Multiskan GO, Shanghai, China). |
| GSH b | Microplate method | |
| GPx c | Colorimetric method | |
| MDA d | TBA method | |
| CAT e | Ammonium molybdenum acid method | |
a
SOD, Superoxide dismutase.
b
GSH, Glutathione.
c
GPx, Glutathione peroxidase.
d
MDA, Malondialdehyde.
e
CAT, Catalase.