Table 3.
Primer sequence for qRT-PCR.
| Target genes | Forward primer (5’-3’) | Reverse primer (5′–3′) | Temperature (°C) | Accession no. |
|---|---|---|---|---|
| β-actin | CGTGACATCAAGGAGAAG | GAGTTGAAGGTGGTCTCAT | 61.4 | M25013 |
| p38 MAPK a | TGGGAGCAGACCTCAACAAT | TACCATCGGGTGGCAACATA | 60.4 | KM112098 |
| TOR b | TCCCACTTTCCACCAACT | ACACCTCCACCTTCTCCA | 61.4 | JX854449 |
| S6K1 c | TGGAGGAGGTAATGGACG | ACATAAAGCAGCCTGACG | 54.0 | EF373673 |
| 4EBP1 d | GCTGGCTGAGTTTGTGGTTG | CGAGTCGTGCTAAAAAGGGTC | 60.3 | KT757305 |
| 4EBP2 e | CACTTTATTCTCCACCACCCC | TTCATTGAGGATGTTCTTGCC | 60.3 | KT757306 |
| NF-kB p65 f | GAAGAAGGATGTGGGAGATG | TGTTGTCGTAGATGGGCTGAG | 62.3 | KJ526214 |
| Nrf2 g | CTGGACGAGGAGACTGGA | ATCTGTGGTAGGTGGAAC | 62.5 | KF733814 |
| Keap1 h | TTCCACGCCCTCCTCAA | TGTACCCTCCCGCTATG | 63.0 | KF811013 |
| IL-4/13A i | CTACTGCTCGCTTTCGCTGT | CCCAGTTTTCAGTTCTCTCAGG | 55.9 | KT445871 |
| IL-4/13B j | TGTGAACCAGACCCTACATAACC | TTCAGGACCTTTGCTGCTTG | 55.9 | KT625600 |
| TGF-β2 k | TACATTGACAGCAAGGTGGTG | TCTTGTTGGGGATGATGTAGTT | 55.9 | KM279716 |
| IFN-γ2 l | TGTTTGATGACTTTGGGATG | TCAGGACCCGCAGGAAGAC | 60.4 | JX657682 |
| TNF-α m | CGCTGCTGTCTGCTTCAC | CCTGGTCCTGGTTCACTC | 58.4 | HQ696609 |
| IL-6 n | CAGCAGAATGGGGGAGTTATC | CTCGCAGAGTCTTGACATCCTT | 62.3 | KC535507.1 |
| IL-8 o | ATGAGTCTTAGAGGTCTGGGTG | ACAGTGAGGGCTAGGAGGG | 60.3 | JN663841 |
| IL-15 p | CCTTCCAACAATCTCGCTTC | AACACATCTTCCAGTTCTCCTT | 61.4 | KT445872 |
| CAT q | GAAGTTCTACACCGATGAGG | CCAGAAATCCCAAACCAT | 58.7 | FJ560431 |
| Cu/Zn-SOD r | CGCACTTCAACCCTTACA | ACTTTCCTCATTGCCTCC | 61.5 | GU901214 |
| GPx-1α s | GGGCTGGTTATTCTGGGC | AGGCGATGTCATTCCTGTTC | 61.5 | EU828796 |
| GPx-4 t | CTGGAGAAATACAGGGGTTACG | CTCCTGCTTTCCGAACTGGT | 60.3 | KU255599 |
a
p38 MAPK, p38 mitogen-activated protein kinase.
b
TOR, Target of rapamycin.
c
S6K1, S6kinase1.
d
4EBP1, 4E binding protein 1.
e
4EBP2, 4E binding protein 2.
f
NF-kB p65, Nuclear factor-kappa B p65.
g
Nrf2, Nuclear factor erythroid 2-related factor 2.
h
Keap1, Kelch-like ECH-associated protein 1.
i
IL-4/13A, Interleukin 4/13A.
j
IL-4/13B, Interleukin 4/13B.
k
TGF-β2, Transforming growth factor β2.
l
IFN-γ2, Interferon γ2.
m
TNF-α, Tumour necrosis factor α.
n
IL-6, Interleukin 6.
o
IL-8, Interleukin 8.
p
IL-15, Interleukin 15.
q
CAT, Catalase.
r
Cu/Zn-SOD, Copper zinc superoxide dismutase.
s
GPx-1α, Glutathione peroxidase 1α.
t
GPx-4, Glutathione peroxidase 4.