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. 2021 Feb 25;12:620238. doi: 10.3389/fimmu.2021.620238

Figure 2.

Figure 2

Caspase-1 activation and IL-1β and IL-18 secretion in macrophages were inhibited in Pin1-/- mice. (A, B) WT and Pin1-/- bone marrow-derived macrophages (BMDMs) were primed with 500 ng/ml lipopolysaccharide (LPS) for 4 h and stimulated with ATP or nigericin for different lengths of time, supernatants were analyzed for IL-1β and IL-18. (C) WT and Pin1-/- BMDMs were primed with 500 ng/ml LPS for 4 h and then stimulated with 5mM ATP or 20 mM nigericin for 30 min. Lysates were immunoblotted for caspase-1. (D) RAW 264.7 cells transfected with control siRNA or siRNA for Pin1 were primed with 500 ng/ml LPS for 4 h and then stimulated with 5 mM ATP or 20 mM nigericin for 30 min. Lysates were immunoblotted for caspase-1. (E) RAW 264.7 cells with high Pin1 expression (Pin1-hi) were primed with 500ng/ml LPS for 4h and then stimulated with 5 mM ATP or 20 mM nigericin for 30 min. Lysates were immunoblotted for caspase-1. The data represent the mean ± SD of one among three biological replicates, with three technical replicates each. *P < 0.05; **P < 0.01, two-way ANOVA (A, B).

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