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. 2021 Feb 25;12:620238. doi: 10.3389/fimmu.2021.620238

Figure 3.

Figure 3

Pin1 regulates NLRP3 expression and transcription in macrophages. (A) Nlrp3, Asc, Casp1, and IL-1β mRNA levels in LPS-treated WT and Pin1-/- bone marrow-derived macrophages (BMDMs). (B) Nlrp3, Asc, Casp1, and IL-1β mRNA levels in lipopolysaccharide (LPS)-treated RAW 264.7 cells transfected with control siRNA or siRNA for Pin1. (C) IL-1b and NLRP3 inflammasome protein levels in LPS-treated WT and Pin1-/- BMDMs after 4 h. (D) IL-1b and NLRP3 inflammasome protein levels in LPS-treated RAW 264.7 cells transfected with control siRNA or siRNA for Pin1 after 4h. (E) RAW 264.7 cells with high Pin1 expression (Pin1-hi) were primed with 500 ng/ml LPS for 4h, lysates were immunoblotted for NLRP3 and ASC. (F, G) WT and Pin1-/- BMDMs were treated without stimulation (control) or with LPS (500ng/ml), ATP (5mM) or nigericin (20mM) alone, LPS for 4 h and 5 mM ATP or 20 mM nigericin for 30 min. The secretion of IL-6 and TNF-α in culture supernatant were analyzed. The data represent the mean ± SD of one among three biological replicates, with three technical replicates each. *P < 0.05; **P < 0.01; ns, no significance; two-way ANOVA (A, B, F, G).

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