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. 2021 Feb 25;12:620238. doi: 10.3389/fimmu.2021.620238

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Copyright © 2021 Dong, Xue, Fan, Zhang, Wang, Li and Da

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pin1 deficiency prevents caspase-1-mediated cleavage of gasdermin D (GSDMD). (A) Imaging assay of pyroptosis in WT and Pin1^-/- bone marrow-derived macrophages (BMDMs) treated with 5 mM ATP or 20 mM nigericin. (B) BMDMs were primed with 500 ng/ml LPS for 4 h and stimulated with ATP or nigericin for 30 min and supernatants were analyzed for lactate dehydrogenase (LDH). (C) Immunoblotting with mouse antibody for GSDMD in WT and Pin1^-/- BMDMs treated with ATP or nigericin. (D) Immunoblotting with mouse antibody for GSDMD in RAW 264.7 cells transfected with control siRNA or siRNA for Pin1 treated with 5 mM ATP or 20 mM nigericin for 30 min. All data are representative of three independent experiments. The data represent the mean ± SD of one among three biological replicates, with three technical replicates each. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance; two-way ANOVA (B).

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