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. 2021 Feb 25;9:625540. doi: 10.3389/fcell.2021.625540

FIGURE 1.

FIGURE 1

Effect of SIRT6 inhibition on meiotic maturation and spindle assembly checkpoint (SAC) activation in porcine oocytes. (A) Representative images of in vitro-matured oocytes in control and SIRT6-inhibited groups. Polar body extrusion (PBE) of denuded oocytes (DOs) and cumulus cell expansion of cumulus cell–oocyte complexes (COCs) were imaged by the confocal microscope. Scale bar, 360 μm (a,d); 240 μm (b,e); 30 μm (c,f). (B) The proportion of PBE was calculated in control and different concentrations of SIRT6-inhibited groups (50 and 100 μM) after 44 h in vitro culture. (C) The localization of BubR1 on the chromosomes in control and SIRT6-inhibited oocytes at pro-metaphase I (M I) and M I stages. Porcine oocytes were immunostained with BubR1 antibody and counterstained with Hoechst. Scale bar, 10 μm. (D) The proportion of BubR1 presence at the M I stage was calculated in control and SIRT6-inhibited oocytes. Data of panels (B,D) were shown as the mean percentage (mean ± SEM) of at least three independent experiments. **P < 0.01, ***P < 0.001.