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. 2021 Feb 25;9:625540. doi: 10.3389/fcell.2021.625540

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Copyright © 2021 Li, Miao, Chen and Xiong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of SIRT6 inhibition on the spindle assembly and chromosome alignment in porcine oocytes. (A) Representative images of spindle/chromosome structure in control and SIRT6-inhibited oocytes. Porcine oocytes were immunostained with α-tubulin-FITC antibody to show the spindle morphologies and were counterstained with propidium iodide (PI) to display the chromosome alignment. Scale bar, 10 μm. (B) The proportion of abnormal spindles was calculated in control and SIRT6-inhibited oocytes. (C) The proportion of misaligned chromosomes was calculated in control and SIRT6-inhibited oocytes. Data of panels (B,C) were expressed as the mean percentage (mean ± SEM) of at least three independent experiments. ***P < 0.001.