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. 2021 Feb 25;9:625540. doi: 10.3389/fcell.2021.625540

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Copyright © 2021 Li, Miao, Chen and Xiong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of SIRT6 inhibition on the localization patterns of cortical granules (CGs) and ovastacin in porcine oocytes. (A) Representative images of CG distribution in control and SIRT6-inhibited oocytes. Porcine oocytes were stained with LCA-FITC to display the CGs. Scale bar, 30 μm. (B) The fluorescence intensity of LCA signals was quantified in control and SIRT6-inhibited oocytes. (C) Representative images of ovastacin distribution in control and SIRT6-inhibited oocytes. Porcine oocytes were immunostained with ovastacin antibody and imaged by confocal microscope. Scale bar, 30 μm. (D) The fluorescence intensity of ovastacin signals was quantified in control and SIRT6-inhibited oocytes. Data of panels (B,D) were expressed as the mean percentage (mean ± SEM) of at least three independent experiments. ***P < 0.001.