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. 2021 Feb 25;9:625540. doi: 10.3389/fcell.2021.625540

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Copyright © 2021 Li, Miao, Chen and Xiong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of SIRT6 inhibition on the levels of DNA damage in porcine oocytes. (A) Representative images of γH2A.X accumulation in control and SIRT6-inhibited oocytes. Porcine oocytes were immunostained with γH2A.X antibody and imaged by confocal microscope. Scale bar, 20 μm. PB1, first polar body. (B) The fluorescence intensity of γH2A.X signals was quantified in control and SIRT6-inhibited oocytes at the metaphase I (M I) stage. (C) The fluorescence intensity of γH2A.X signals was quantified in control and SIRT6-inhibited oocytes at the M II stage. Data of panels (B,C) were expressed as the mean percentage (mean ± SEM) of at least three independent experiments. ***P < 0.001.