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Effect of SIRT6 inhibition on the early apoptosis in porcine oocytes. (A) Representative images of apoptotic oocytes by Annexin-V staining in control and SIRT6-inhibited groups. Scale bars, 40 and 80 μm. (B) The proportion of apoptotic oocytes was calculated in control and SIRT6-inhibited groups. Data were expressed as the mean percentage (mean ± SEM) of at least three independent experiments. ***P < 0.001.
