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. 2021 Feb 25;9:616893. doi: 10.3389/fcell.2021.616893

FIGURE 4.

FIGURE 4

Endometrial stromal cells (EndoSCs) increase collagen I and ED-A fibronectin production in response to transforming growth factor β1 (TGF-β1) treatment with a distinct pattern of extracellular matrix (ECM) deposition. (A) Representative Western blotting of EndoSC, dermal stromal cell (DermSC), and adipose tissue stromal cell (AdipoSC) lysates obtained before TGF-β1 treatment (lane 0), at 96 h in control culture (lane 96/–), or at 96 h after addition of 10 ng/ml TGF-β1 (lane 96/ +). (B) Densitometry analysis of ECM components in stromal cells shown in panel (A) at experimental endpoint (96 h). Individual donor data (n = 3) presented as fold change relative to the level before TGF-β1 treatment (logarithmic scale) with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control used for normalization. Paired t-test was used for statistical analysis of log-transformed data; ∗p < 0.05. (C) Immunofluorescence of collagen I and ED-A fibronectin in EndoSC, DermSC, and AdipoSC cultures treated with TGF-β1 and untreated control. Scale bar 100 μm. (D) Higher level of collagen I and ED-A fibronectin colocalization in EndoSC than in DermSC and AdipoSC in control and TGF-β1-treated cultures; Pearson’s correlation coefficient for three repeats, one-way ANOVA and Newman–Keuls test were used for statistical analysis; ∗p < 0.05.