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. 2021 Feb 25;9:616893. doi: 10.3389/fcell.2021.616893

FIGURE 5.

FIGURE 5

Stromal cells from the endometrium (EndoSCs), dermis (DermSCs), and adipose tissue (AdipoSCs) acquired myofibroblast phenotype after transforming growth factor β1 (TGF-β1) treatment. (A) Western blotting analysis of α-smooth muscle actin (α-SMA) and vinculin in EndoSC, DermSC, and AdipoSC lysates obtained before TGF-β1 (lane 0), at 96 h in control culture (lane 96/–), or at 96 h after addition of 10 ng/ml TGF-β1 (lane 96/+). (B) Densitometry analysis of α-SMA changes in stromal cells treated by TGF-β1 shown in panel (A). Individual donor data (n = 3) presented as fold change relative to the level of the evaluated protein before TGF-β1 treatment (logarithmic scale) with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control used for normalization. Paired t-test was used for statistical analysis of log-transformed data; ∗p < 0.05. (C) Immunofluorescence of α-SMA in EndoSC, DermSC, and AdipoSC cultures treated with TGF-β1 and in untreated control. Scale bar 100 μm. (D) Immunofluorescence of vinculin in EndoSC, DermSC, and AdipoSC cultures treated with TGF-β1 and in untreated control. The same field of view as in panel (C) is presented. Scale bar 100 μm. (E) Merged high-magnification images after immunofluorescent labeling of α-SMA and vinculin in EndoSC, DermSC, and AdipoSC cultures treated by TGF-β1. Scale bar 100 μm.