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. 2021 Feb 25;9:616893. doi: 10.3389/fcell.2021.616893

FIGURE 7.

FIGURE 7

Local factors produced during menstruation inhibit phenotypic transition to myofibroblasts in stromal cells from the endometrium (EndoSCs), dermis (DermSCs), or adipose tissue (AdipoSCs). (A) Representative Western blotting of α-smooth muscle actin (α-SMA) and vinculin in lysates of EndoSC, DermSC, and AdipoSC treated with 10% venous blood serum (BS), menstrual discharge serum (MDS), or fetal bovine serum (FBS) at 96 h of experiment. (B) Densitometry of α-SMA and vinculin changes in stromal cells treated by serum preparations shown in panel (A). Individual donor data (n = 3) presented as fold change relative to the evaluated protein level in control sample treated with FBS (logarithmic scale); glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control used for normalization. Paired t-test was used for statistical analysis of log-transformed data; *p < 0.05. (C) Immunofluorescence of α-SMA and vinculin in EndoSC, DermSC, and AdipoSC treated with 10% BS, 10% MDS, or 10%FBS; 96 h of experiment. Scale bar 100 μm. (D) Concentrations of transforming growth factor β1 (TGF-β1) in MDS, BS, and FBS measured by ELISA. Mann–Whitney test was used for statistical analysis; *p < 0.05.