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. 2021 Feb 25;15:646413. doi: 10.3389/fncel.2021.646413

FIGURE 1.

FIGURE 1

Expression of the Olfr17 alleles in different genetic backgrounds. (A) Number of olfactory sensory neurons expressing Olfr17 in the wild type mouse strains. Coronal sections through the anterior-posterior axis of the olfactory epithelium from 129 and B6 mouse strains were hybridized with antisense digoxigenin-labeled probe specific for Olfr17. Representative images of the labeled sections are shown. The graph shows the number of Olfr17-positive neurons per olfactory epithelium area. N = 3 animals from each strain, five sections per animal. Student’s t-test ***P < 0.001. (B) RT-qPCR was performed to determine the relative Olfr17 gene expression levels in the olfactory epithelium from 129 and B6 wild type mouse strains, or from P2-GFP− /− and P2-GFP+/+ knock-in siblings. The levels were normalized to β-actin and are shown relative to the expression in the wild type B6 olfactory epithelium. The graph shows mean +/− SEM (B6, n = 3; 129, n = 4; P2-GFP− /−, n = 4; P2-GFP+/+, n = 4). Not significant: ns, *P < 0.05; **P < 0.01 and ***P < 0.001. One-way ANOVA followed by Newman–Keuls post hoc test. (C) Coronal sections through the anterior-posterior axis of the olfactory epithelium from P2-GFP− /− and P2-GFP+/+ mice were hybridized with antisense digoxigenin-labeled probe specific for Olfr17. Olfactory neurons that hybridized with the Olfr17 probe were counted in 7–10 adjacent sections from each genotype from the three indicated locations using Tissue FAXS. Representative images of the labeled sections are shown on the top right. The number of labeled neurons (black dots) per olfactory epithelium area (labeled in green), was determined in each section. Student’s t-test, *P < 0.05 and **P < 0.01. Dots and squares represent the number of Olfr17-positive neurons per olfactory epithelium area in each section.