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. 2021 Feb 25;12:643472. doi: 10.3389/fmicb.2021.643472

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Copyright © 2021 Zhou, Bi, Ding and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fermentation of the strains with multiple-exogenous-module optimization. (A) The VC production of the initial strain (YLAA, gray), the strain overexpressing the whole exogenous module (YLAA-2, red), the strain overexpressing different fusion-protein (YLAAF-1/2/3, blue), and the strain overexpressing different combination modules (YLAAK-1/2/3/4, green) after 72 h of fermentation. (B) Time courses showing changes in cell concentration of YLAAK-1 (triangles), YLAAK-2 (rhombuses), YLAAK-3 (hexagons), YLAAK-4 (squares) and BY4741 (circles). Error bars represent ± SEM (n = 3). Significance levels of Student’s t-test: ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^****P < 0.0001.