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. 2021 Feb 25;11:627043. doi: 10.3389/fcimb.2021.627043

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Copyright © 2021 Amaya Arbeláez, de Paula e Silva, Navegante, Valente, Barbugli and Vergani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell cycle distribution index. NOK-si (A), SCC 25 (B), and Detroit 562 (C) cell lines stimulated with soluble factors (SF) from C. albicans (Ca), S. aureus (Sa), and dual species (D) from early (16-h) (A, C, E) and mature (36-h) (B, D, F) biofilms. Cells were fixed and labeled with propidium iodide, and their DNA content was measured using flow cytometry. The cell cycle distribution index (SubG0, G0/G1, S, G2/M) was analyzed after 4 and 24 h of stimulation.