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. 2021 Feb 25;11:627043. doi: 10.3389/fcimb.2021.627043

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Copyright © 2021 Amaya Arbeláez, de Paula e Silva, Navegante, Valente, Barbugli and Vergani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proto-oncogenes and cell cycle gene expression upon SF from mature biofilms. Gene expression was evaluated by the RT-qPCR analysis of NOK-si, SCC 25, and Detroit 562 upon 4 h of stimulation with soluble factors (SF) of C. albicans (Ca) (□), S. aureus (Sa) (•), and dual species (D) (Δ) from mature 36-h biofilms. Data are shown as fold change values compared with cells stimulated with RPMI-1640 medium (control – black line) supplemented using the 2^−ΔΔCt method (HPRT was used as internal control). *IC 95% means a significant difference vs. the control.