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. 2021 Feb 25;9:636498. doi: 10.3389/fcell.2021.636498

FIGURE 5.

FIGURE 5

Withaferin A and ovarian cancer’s effect on the UPR at the protein level. (A) Representative immunoblots for components of the PERK and IRE1α arms of the UPR in QF muscle samples in the tumor-free and tumor-bearing vehicle-treated and WFA 4 mg/kg groups. N = 3 in all groups. (B) Densitometric quantification of western blot results. (C) Representative images of spliced (sXBP-1), unspliced (uXBP-1) and total XBP-1 levels in GA muscle samples from tumor-free and tumor-bearing animals. Unrelated gene (β-Actin) was used as a load control for normalization. N = 3 per group. (D) Densitometric quantification of transcript levels of XBP-1 and β-Actin. N = 3 per group. “n.s.” = no significant differences. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, value significantly different from corresponding value of tumor-free vehicle-treated group by two-way ANOVA followed by Tukey’s multiple comparison test post hoc analysis. @p < 0.05, value significantly different from corresponding value of tumor-bearing vehicle-treated group.