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. 2021 Feb 25;12:643692. doi: 10.3389/fimmu.2021.643692

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Copyright © 2021 Yuan, Wang, Huang, Shangguan and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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State of the art of single-cell technology and its application in breast cancer studies. Single-cell sequencing technologies have been designed for almost all the molecular layers of genetic information flow from RNA to proteins. For each molecular layer, multiple technologies have been developed, all of which have specific advantages and disadvantages. Single-cell technologies are close to comprehensively depicting the state of the functional properties and dynamic changes of immune cells in the tumor microenvironment.