Figure 7.

Expression profile of immune cell lineage and subset marker in Ly6C MC subset. (A) Expression pattern of newly suggested leukocyte signature genes in Ly6C MC. Heatmap shows the expression levels of the leukocyte signature genes, recently suggested by scRNA-seq study46, 47 in Ly6C MC. The color density indicates the average expression of a given gene normalized by z-score. Fold change of newly suggested leukocyte signature gene are present in the Supplementary Table 5 . (B) Association of Ly6C MC with newly suggested leukocyte subset signature genes. Connection of the newly suggested leukocyte signature genes with Ly6C MC subsets are established based on their expression pattern in Ly6C MC subsets. (C) Expression profile of 58 SDE established lineage surface markers in Ly6C MC. (D) Expression profile of 38 SDE established lineage transcription factors in Ly6C MC. Four major immune cell type (MΦ/DC/TC/BC) and their 15 subsets are listed. Lineage SDE surface markers and TF are differentially expressed in four comparison groups in these subsets. Red-colored background numbers indicate FC>2 (log₂FC>1). Green-colored background numbers indicate FC<0.5 (log₂FC<-1). Justification for Leukocyte lineage specific TF/surface marker are listed in the Supplementary Table 6 . scRNA-seq, single-cell RNA sequencing; MC, monocyte, Cbs, Cystathionine β-synthase; MΦ,macrophage; DC, dendritic cell; MoDC monocyte-derived dendritic cell; TC, T cell, T_h2 cell, T helper 2 cell; BC, B cell; APC, antigen-presenting cells; TF, transcription factor; cDC, classical DCs, pDC, plasmacytoid DC; Treg, Regulatory T cells; Tfh, T follicular helper.