Skip to main content
. 2021 Feb 25;15:628983. doi: 10.3389/fnins.2021.628983

FIGURE 4.

FIGURE 4

Changes in the incidence of GFAP-, PDGFRα-, and DCX-positive cells after Wnt signaling manipulation in intact and ischemic mice. The incidence of cells showing passive (pas; GFAP+ cells), complex (com; PDGFRα+ cells) or outwardly rectifying (out; DCX+ cells) current patterns was examined in neural stem/progenitor cell (NS/PC) cultures derived from adult control, non-operated (CTRL; A) as well as operated (MCAO; B) mice. The relative incidence of cells in controls was arbitrarily set to 1. Sixteen founder mice were used to derive NS/PCs from each mouse strain. The incidence was quantified from the following total number of cells (in brackets): CTRL-dnTCF4-EtOH (145), CTRL-dnTCF4-4OHT (141), CTRL-Dkk1-EtOH (116), CTRL-Dkk1-4OHT (114), CTRL-Ex3-EtOH (152), CTRL-Ex3-4OHT (197), MCAO-dnTCF4-EtOH (75), MCAO-dnTCF4-4OHT (72), MCAO-Dkk1-EtOH (75), MCAO-Dkk1-4OHT (79), MCAO-Ex3-EtOH (80), and MCAO-Ex3-4OHT (80). The incidence of cells in controls (EtOH) was compared to the incidence of the same cell types in cells with manipulated Wnt signaling (4OHT) with Student’s t-test. **p < 0.01. 4OHT, (Z)-4-hydroxytamoxifen; DCX, doublecortin; Dkk1, Dickkopf 1; dnTCF4, dominant negative T-cell factor 4; EtOH, ethanol; Ex3, exon 3; GFAP, glial fibrillary acidic protein; MCAO, middle cerebral artery occlusion; PDGFRα, platelet-derived growth factor receptor alpha.