TABLE 2.
Membrane properties of differentiated adult NS/PCs isolated from CTRL mice, and showing a passive current profile.
|
dnTCF4 |
Dkk1 |
Ex3 |
||||
| EtOH | 4OHT | EtOH | 4OHT | EtOH | 4OHT | |
| VM [mV] | −84.50.7 | −83.01.0 | −86.80.9 | −85.51.0 | −85.4 ± 0.7 | −87.6 ± 0.6* |
| IR [MΩ ] | 67.64.2 | 66.72.8 | 49.1 ± 3.1 | 75.3 ± 4.5*** | 89.45.0 | 79.24.0 |
| CM [pF] | 27.12.3 | 30.42.9 | 31.4 ± 2.8 | 21.7 ± 1.7** | 22.61.8 | 20.71.4 |
| KIR/CM [pA/pF] | 2.90.3 | 2.50.4 | 2.40.7 | 3.20.4 | 3.50.9 | 2.70.3 |
| KDR/CM [pA/pF] | 4.00.8 | 6.31.5 | 7.42.3 | 8.73.0 | 5.81.0 | 3.90.6 |
| n | 62 | 55 | 25 | 25 | 61 | 63 |
4OHT, (Z)-4-hydroxytamoxifen; CM, membrane capacitance; CTRL, control, non-operated mice; Dkk1, Dickkopf 1; dnTCF4, dominant negative T-cell factor 4; EtOH, ethanol; Ex3, exon 3; IR, input resistance; KDR, delayed outwardly rectifying K+ currents; KIR, inwardly rectifying K+ currents; KIR/CM, KDR/CM, current densities; n, number of cells; NS/PCs, neural stem/progenitor cells; VM, membrane potential. Values in bold indicate significant differences between EtOH- and 4OHT-treated cultures; *p < 0.05, **p < 0.01, ***p < 0.001.