TABLE 4.
Membrane properties of differentiated adult NS/PCs isolated from CTRL mice, and showing a complex current profile.
|
dnTCF4 |
Dkk1 |
Ex3 |
||||
| EtOH | 4OHT | EtOH | 4OHT | EtOH | 4OHT | |
| VM [mV] | −82.9 ± 1.0 | −88.6 ± 0.7*** | −89.00.9 | −86.90.9 | −88.8 ± 0.8 | −86.5 ± 0.7* |
| IR [MΩ ] | 138.212.8 | 153.013.8 | 136.49.7 | 136.39.0 | 175.6 ± 14.0 | 138.6 ± 7.7* |
| CM [pF] | 16.9 ± 1.6 | 12.7 ± 0.8* | 14.51.1 | 15.21.3 | 15.71.2 | 16.61.1 |
| KIR/CM [pA/pF] | 6.10.6 | 7.70.9 | 8.30.8 | 10.71.2 | 7.9 ± 0.7 | 4.5 ± 0.5*** |
| KDR/CM [pA/pF] | 34.95.1 | 52.89.0 | 54.16.3 | 76.09.6 | 42.1 ± 5.5 | 25.6 ± 2.9** |
| KA/CM [pA/pF] | 22.34.3 | 20.83.7 | 45.4 ± 6.3 | 25.1 ± 2.7* | 19.24.3 | 11.42.4 |
| Na+/CM [pA/pF] | 28.212.4 | 39.55.4 | 11.85.1 | 52.90.0 | 19.73.5 | 27.35.7 |
| n | 43 | 45 | 39 | 38 | 45 | 58 |
4OHT, (Z)-4-hydroxytamoxifen; CM, membrane capacitance; CTRL, control, non-operated mice; Dkk1, Dickkopf 1; dnTCF4, dominant negative T-cell factor 4; EtOH, ethanol; Ex3, exon 3; IR, input resistance; KA, fast activating and inactivating outwardly rectifying K+ currents; KDR, delayed outwardly rectifying K+ currents; KIR, inwardly rectifying K+ currents; KIR/CM, KDR/CM, KA/CM, Na+/CM, current densities; n, number of cells; NS/PCs, neural stem/progenitor cells; VM, membrane potential. Values in bold indicate significant differences between EtOH- and 4OHT-treated cultures; **p < 0.01, ***p < 0.001.