TABLE 6.
Membrane properties of differentiated adult NS/PCs isolated from CTRL mice, and showing an outwardly rectifying current profile.
|
dnTCF4 |
Dkk1 |
Ex3 |
||||
| EtOH | 4OHT | EtOH | 4OHT | EtOH | 4OHT | |
| VM [mV] | −85.5 ± 1.6 | −79.4 ± 1.8* | −86.9 ± 1.3 | −81.8 ± 1.2** | −86.2 ± 1.5 | −77.8 ± 1.8*** |
| IR [MΩ ] | 997.197.3 | 887.596.0 | 959.7 ± 97.2 | 352.4 ± 30.2*** | 1217.6 ± 134.3 | 877.8 ± 91.9* |
| CM [pF] | 8.40.8 | 9.50.6 | 9.1 ± 0.5 | 16.1 ± 1.2*** | 9.1 ± 0.6 | 11.1 ± 0.8* |
| KDR/CM [pA/pF] | 112.27.1 | 118.39.0 | 139.68.9 | 150.514.1 | 101.24.7 | 88.55.8 |
| KA/CM [pA/pF] | 84.57.6 | 75.512.3 | 110.9 ± 9.8 | 40.1 ± 7.2*** | 80.6 ± 7.3 | 44.0 ± 7.1*** |
| Na+/CM [pA/pF] | −− | 24.212.8 | 9.8 ± 1.1 | 19.1 ± 4.3* | 12.42.6 | 14.44.3 |
| n | 47 | 47 | 65 | 57 | 62 | 61 |
4OHT, (Z)-4-hydroxytamoxifen; CM, membrane capacitance; CTRL, control, non-operated mice; Dkk1, Dickkopf 1; dnTCF4, dominant negative T-cell factor 4; EtOH, ethanol; Ex3, exon 3; IR, input resistance; KA, fast activating and inactivating outwardly rectifying K+ currents; KDR, delayed outwardly rectifying K+ currents; KDR/CM, KA/CM, Na+/CM, current densities; n, number of cells; NS/PCs, neural stem/progenitor cells; VM, membrane potential. Values in bold indicate significant differences between EtOH- and 4OHT-treated cultures; *p < 0.05, **p < 0.01, ***p < 0.001.