TABLE 7.
Membrane properties of differentiated adult NS/PCs isolated from MCAO mice, and showing an outwardly rectifying current profile.
|
dnTCF4 |
Dkk1 |
Ex3 |
||||
| EtOH | 4OHT | EtOH | 4OHT | EtOH | 4OHT | |
| VM [mV] | −77.4 ± 2.4 | −85.7 ± 1.4* | −78.12.3 | −84.32.1 | −80.1 ± 2.5 | −67.0 ± 3.4** |
| IR [MΩ ] | 1112.2132.8 | 1028.2123.1 | 1166.2157.2 | 741.8132.1 | 1230.8228.2 | 1018.7156.6 |
| CM [pF] | 8.90.4 | 8.80.3 | 9.00.4 | 7.91.3 | 10.21.1 | 9.10.8 |
| KDR/CM [pA/pF] | 100.4 ± 5.3 | 149.3 ± 19.5** | 109.56.7 | 137.117.1 | 99.36.6 | 114.610.9 |
| KA/CM [pA/pF] | 80.86.2 | 95.512.1 | 103.7 ± 10.1 | 180.3 ± 30.1** | 87.512.3 | 94.919.4 |
| Na+/CM [pA/pF] | 11.60.3 | 15.72.3 | 12.35.5 | − | − | 31.910.4 |
| n | 34 | 19 | 37 | 13 | 18 | 24 |
4OHT, (Z)-4-hydroxytamoxifen; CM, membrane capacitance; Dkk1, Dickkopf 1; dnTCF4, dominant negative T-cell factor 4; EtOH, ethanol; Ex3, exon 3; IR, input resistance; KA, fast activating and inactivating outwardly rectifying K+ currents; KDR, delayed outwardly rectifying K+ currents; KDR/CM, KA/CM, Na+/CM, current densities; MCAO, middle cerebral artery occlusion; n, number of cells; NS/PCs, neural stem/progenitor cells; VM, membrane potential. Values in bold indicate significant differences between EtOH- and 4OHT-treated cultures; *p < 0.05, **p < 0.01.