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. 2020 Nov 3;38(3):1122–1136. doi: 10.1093/molbev/msaa279

Fig. 3.

Fig. 3.

Functional consequences of ASIP over-expression in white buffalo skin. (A) Transcription levels of ASIP, TRY, DCT (TYRP2), TYRP1, KIT, KITLG, MITF, MC1R, and EDNRB. Error bars indicate standard deviation (SD). “**”Significance level (P < 0.01) of a Wald test using the DESeq2 package. (B) Immunohistochemical analysis of skin samples of black (top) and white (bottom) buffaloes. Arrowheads indicate melanocytes with tyrosinase-related protein 1 (TYRP1) expression (Trp+). (C) Hematoxylin−Eosin (HE) staining of skin samples of black (top) and white (bottom) buffaloes. Arrowheads indicate melanin pigment near the melanocytes.