FIGURE 1.

Peptidoglycan-binding anchor (Pba) is expressed by clinical strains and is absent in the Pseudomonas aeruginosa PW2884 mutant. Pba expressions of the reference strain PAO1, four clinical P. aeruginosa strains, the mutant PW2884 with a transposon insert in pba, and the corresponding wild-type strain (MPAO1). Bacterial outer membrane proteins (OMPs) and recombinantly expressed Pba were separated on SDS-PAGE and silver stained (A). Pba was detected in western blot (B) from a corresponding gel with rabbit anti-Pba pAbs. PAO1, transposon mutant PW2884, and a transcomplemented strain (PW2884 + pba) were further analyzed for Pba content from whole cell lysates and purified OMVs in silver-stained SDS-PAGE (C) and Western blot (D).